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Non-viral transfection agents are essential components in RNA-based therapeutics and diagnostics. Consider for example, that the recently developed vaccines for preventing COVID-19 caused by SARS-CoV-2 rely on lipid nanoparticle (LNP) formulations that stabilize mRNA and promote its desired immune response. While LNPs have been largely successful in the control of the COVID-19 pandemic, there are still many issues to improve on, including off-target effects, stability, and toxicity. Conjugated oligoelectrolytes (COE) are defined by a linear hydrophobic conjugated backbone with polar ionic pendants at both terminals. The modular design of COEs allows for rapid tuning of molecular units to access a large continuum of properties. They were first synthesized as two-photon absorption chromophores and later extended to several biological applications such as antimicrobial agents and extracellular vesicle dyes.

In this project, we are interested in developing novel LNP formulations incorporating COEs with a view toward therapeutic applications. Routine assays will be conducted on the LNP, including particle characterizations (DLS, cryo-TEM, and encapsulation efficiency), transfection, cellular uptake, confocal imaging of intracellular fates, and in vivo delivery. The performance of the novel LNP formulations will be routinely compared to commercial agents.

This project will be closely mentored by a postdoctoral research fellow and principal investigator.

Job Requirements

• The candidate must have a minimum of honours or its equivalent
• Independent and motivated individual with good problem-solving skills
• With practical experience and/or knowledge of:
  • Biological chemistry, cell biology, or biochemistry
  • Biomaterials/materials science
  • Characterization techniques including absorbance/fluorescence measurements, flow cytometry, or confocal microscopy 
  • Mammalian cell culture techniques
  • Animal work experience
  • Knowledge of lipid nanoparticles and drug/gene delivery is highly sought after
  • Microsoft Office

More Information

Location: Kent Ridge Campus
Organization: Institute for Functional Intelligent Materials
Department: Research Groups
Job requisition ID: 30973