Job Description

Chronic Hepatitis B (CHB) remains a major global health challenge, and functional cure, defined by HBsAg loss and immune restoration , is rarely achieved.

Our multidisciplinary program integrates clinical cohorts with mechanistic laboratory studies to uncover how hepatocytes transition from a pro-viral tolerant state to an immune-reactive flare state that precedes viral clearance.

This research aims to identify molecular triggers and therapeutic targets that drive immune-mediated HBV flares, combining hepatocyte biology, RNA therapeutics, and spatial transcriptomics.

Key Responsibilities

• Perform cell culture and molecular assays (HepAD38, HepG2, Huh7, primary hepatocytes, and co-culture systems).
• Conduct HBV replication and gene expression analyses (RNA/DNA extraction, qPCR, ELISA, Western blotting).
• Assist with RNA therapeutics and GalNAc-ASO delivery studies in hepatocyte models.
• Maintain meticulous experimental records, reagent inventories, and safety documentation.
• Support data analysis and visualization (Excel, GraphPad, or Python/R if skilled).
• Liaise with clinical teams and collaborators on translational aspects of the project.

Qualifications

• Bachelor’s degree in Life Sciences, Biomedical Sciences, or Molecular Biology (recent graduates are welcome).
• Prior experience in cell culture and molecular biology is essential.
• Experience with hepatocyte or viral systems (HBV, HCV, etc.) is an advantage.
• Strong work ethic, curiosity, and ability to learn new techniques quickly.
• Excellent organizational, communication, and teamwork skills.

Training & Opportunities

• Exposure to cutting-edge platforms: RNA therapeutics, AAV-HBV1.2 mouse models, and spatial transcriptomics (CosMx).
• Opportunity to contribute to high-impact publications and national translational HBV consortia.
• Ideal stepping stone for candidates aiming for PhD training or biomedical research careers.

More Information

Location: Kent Ridge Campus

Organization: Yong Loo Lin School of Medicine

Department : Medicine

Employee Referral Eligible: No

Job requisition ID : 31056

Contact list for further enquiries

Hiring Manager: Amy Tay <mdctyla@nus.edu.sg>Lim Seng Gee

Hiring Manager Email: MDCLIMSG@nus.edu.sg

HR Partner: Poong Nyuk Jing, Haley

HR Partner Email: HALEYPNJ@nus.edu.sg