Job Description

A full-time Research Assistant/Research Associate in the Low laboratory is available starting effective immediately. The Low laboratory will study immunology at the interplay between cancer and infectious diseases, with a particular focus on T and B cell biology. For more information, please check out our lab website at www.junsionglow.com

Duties & Responsibilities

a)    Laboratory

• Ensure that the laboratory is adequately stocked with clean glassware, chemicals, and consumables for experiments
• Responsible for the cleanliness and maintenance of equipment and the laboratory
• To assist in making buffers and restocking of consumables as required for lab members
• To ensure laboratory plasmids, cell lines and consumables are properly stored
• Maintain an up-to-date inventory and database of existing lab materials, consumables and plasticware in the lab and order as necessary

b)    Research

• To help in carrying out assigned Molecular Biology and Cellular Biology experiments such as
  
  • Plasmid cloning and PCR
  • Genetic manipulation experiments in cells using lentiviruses or CRISPR-Cas9 system
  • Harvest protein, RNA, or DNA from tissue, cell lines, and bacteria
  • Measure protein or RNA level changes by Western blot, immunostaining, qRT-PCR
  • Monitor changes cellular phenotypes by fluorescent microscopy and flow cytometry
  • Long term tissue culture 
  • Processing of patient blood and tissue samples 
  • Protein production and purification
• To organize, document and present the results of the experiments in a logical manner
• To assist in supervising, training, and guiding new graduate and undergraduate students

Requirements

Requirements 

• University degree (biology, biomedical science, immunology, molecular biology, biochemistry, genetics, or related area)
• Ability to speak and write well in English
• Good organization skills and sterility in lab practices

Knowledge of:

• Basic principles and practices in the fields of molecular, cancer and immunobiology
• General rules for safe exposure and handling of chemical and biological hazards
• Appropriate computer operations, such as Microsoft Word, Excel, PowerPoint, ImageJ (FIJI plugin), adobe photoshop, Illustrator and Flowjo.

Interested applicants should send the following documents in the job application to lowjs@nus.edu.sg